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KMID : 0363620170380030043
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Journal of Korean Oriental Medicine
2017 Volume.38 No. 3 p.43 ~ p.58
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Inhibition of gene associated with Dyslipidemia and Antioxidative Effect of Artemisia iwayomogi, Curcumae Radix and Raphani Semen(ACR) on HepG2 cell model
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Cha Ji-Yun
Yoo Ho-Rhyong
Kim Yoon-Sik
Seol In-Chan
Cho Hyun-Kyoung
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Abstract
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Objectives: We performed this study to evaluate the antioxidative and hypolipidemic effect of Artemisia iwayomogi (韓茵蔯), Curcuma longa L. (鬱金) and Raphanus sativus L. (蘿?子) (ACR).
Method: We enriched Artemisiae Capillaris, Curcumae Longae and Raphani Semen compound with alcohol. ACR extract is treated to HepG2 cell. Cell groups are devided into 3 groups: normal, control and ACR treated group. We measured polyphenol, flavonoids, DPPH and ABTS radical scavenging activity, ROS, glutathione, GSH peroxidase, GSH reductase, SOD, catalase, free fatty acid, lipid peroxidation and suppression of ACAT1 and HMG-CoA reductase expression on mRNA level.
Results:
1. ACR contained polyphenol and flavonoids and increased GSH significantly in HepG2 cell.
2. ACR increased GPx, GR, and catalase activity significantly in HepG2 cell.
3. ACR increased DPPH and ABTS radical scavenging activity significantly in HepG2 cell and decreased ROS.
4. ACR decreased free fatty acid and MDA significantly in HepG2 cell.
5. ACR suppressed ACAT1 and HMG-CoA reductase expression on mRNA level in HepG2 cell.
Conclusion: This study suggests that ACR has antioxidative and hypolipidemic effect and might be effective in prevention and treatment of dyslipidemia.
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KEYWORD
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Dyslipidemia, Hyperlipidemia, Antioxidant, Artemisia iwayomogi, Curcumae Radix, Raphani Semen
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